| Registro completo |
| Provedor de dados: |
Ciência Rural
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| País: |
Brazil
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| Título: |
Presence of multiple acid phosphatases activity in seedlings of cucumber, radish and rocket salad
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| Autores: |
Tabaldi,Luciane Almeri
Ruppenthal,Raquel
Pereira,Luciane Belmonte
Cargnelutti,Denise
Gonçalves,Jamile Fabbrin
Morsch,Vera Maria
Schetinger,Maria Rosa Chitolina
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| Data: |
2008-06-01
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| Ano: |
2008
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| Palavras-chave: |
Cucumis sativus
Raphanus sativus
Eruca vesicaria
Phosphatases
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| Resumo: |
Acid phosphatases (3.1.3.2) are a group of enzymes widely distributed in nature, which catalyze the hydrolysis of a variety of phosphate esters in the pH range of 4-6. We confirmed the presence of acid phosphatases in seedlings of cucumber (Cucumis sativus), radish (Raphanus sativus) and rocket salad (Eruca vesicaria) under different assay conditions using a rapid and simple preparation. The results showed that the optimum pH and temperature used for all species were close to 5.5 and 35°C, respectively. The enzyme was inhibited by molybdate, fluoride, azide, levamisole, orthovanadate, Zn2+ and Cu2+. Suramin had no effect on enzyme activity. The acid phosphatase from cucumber, radish and rocket salad hydrolyzed a wide variety of phosphate esters and the highest activity was observed with PPi, ATP and GTP. These results demonstrate that the enzyme investigated in this study is different from well known ester phosphate cleaving plant enzymes (apyrase and inorganic pyrophosphatases) and this preparation could be a useful tool to future toxicological studies and to study initially all isoforms of acid phosphatase.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782008000300009
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| Editor: |
Universidade Federal de Santa Maria
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| Relação: |
10.1590/S0103-84782008000300009
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| Formato: |
text/html
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| Fonte: |
Ciência Rural v.38 n.3 2008
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| Direitos: |
info:eu-repo/semantics/openAccess
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